rabbit polyclonal anti- col1 antibody  (Novotec Medical GmbH)

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Inhibiting Lysyl Oxidases prevents pathologic cartilage calcification.

doi: 10.1016/j.biopha.2023.116075

Figure Lengend Snippet: Fig. 6. Proposed mechanism based on the observed results. Increased LOX(L) expression and activity promotes cartilage calcification (Anx, Enpp1, Pit1, Pit2, ALP). Both classical LOX(L) role (matrix cross-links) and non-classical roles are involved. The latter encompass: increased chondrocyte hypertrophy (Runx2, COL10), increased fibrosis (Col1, Col3), increased inflammation (IL-6) and increased oxidative stress (ROS). Finally, cartilage calcification can in turn induce increased LOX (L) activity.

Article Snippet: Collagen 1 (COL1) and Collagen 3 (COL3) expression were evaluated using an anti-COL1 rabbit polyclonal antibody (Boster, PA2140–2) and an anti-COL3 rabbit polyclonal antibody (Origene, AP06517PU-N) respectively.

Techniques: Expressing, Activity Assay

Journal: Scientific Reports

Article Title: Doxorubicin-induced modulation of TGF-β signaling cascade in mouse fibroblasts: insights into cardiotoxicity mechanisms

doi: 10.1038/s41598-023-46216-7

Figure Lengend Snippet: DOX inhibited the TGF-β1 induced expression of COL1 in NIH3T3 cells but not in CFs. Western blot analysis of NIH3T3 cells ( A ) and CFs ( B ) probing COL1 protein levels with and without TGF-β1 stimulation and DOX. Densitometry results were normalized to the treatment of TGF-β and without DOX. Values represent mean ± standard error (n = 4). *, P -value < 0.05 compared to treatment stimulated with TGF- β1 without DOX (Student’s t-test). #, P -value < 0.05 compared to treatment group stimulated with TGF-β1 and DOX (Student’s t-test).

Article Snippet: Primary antibodies used in this study were: recombinant rabbit anti-cellular communication network factor 2 (CCN2) monoclonal antibody diluted to a concentration of 1:1,000 (Abcam, ab209780); rabbit anti-bone morphogenetic protein 1 (BMP1) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; PA5-103,660; RRID: AB_2852994); rabbit anti-collagen type I (COL1) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; PA1-26204; RRID: AB_2260734); rabbit anti-suppressors of mothers against decapentaplegic homolog 2 (SMAD2) polyclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; 51–1300; RRID: AB_2533896); rabbit anti-phosphorylated SMAD2 (pSMAD2) monoclonal antibody diluted to a concentration of 1:1,000 (Thermo Fisher Scientific; MA5-15122; RRID: AB_10978317); and mouse anti-beta actin (β-actin) monoclonal antibody diluted to a concentration of 1:5,000 (Thermo Fisher Scientific; MA1-91399; RRID: AB_2273656).

Techniques: Expressing, Western Blot

Journal: iScience

Article Title: Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH

doi: 10.1016/j.isci.2023.107201

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Col1 , Proteintech , Cat# 14695-1-AP; RRID: AB_2082037.

Techniques: Staining, Recombinant, Cell Counting, Enzyme-linked Immunosorbent Assay, Western Blot, Software